Users can run three types of prediction.
Single Prediction gives single ∆∆G for given antibody-antigen structure and information about single mutation.
Multiple Prediction allows to predict ∆∆G for multiple mutations with list of mutations.
Also, users are able to conduct Alanine Scanning or Saturation Mutagenesis for antibody-antigen interface residues.
The result page of Single Prediction is divided into three sections.
The Result section gives predicted ∆∆G, mutation information and antibody information.
The Visualisation part shows inter-/intramolecular interactions of wild-type and mutant structures. Using control panel, users are able to turn on/off each interaction and change the representation of antibody-antigen structure.
The antibody sequence and its Chothia annotation are shown in the Sequence Viewer.
Users are able to download results and interaction files through the Donwload section.
The predicted ∆∆G and corresponding information are shown in the Result section.
The antibody-antigen complex can be coloured by average ∆∆G if any of mutations occurs more than once at the same position.
All the predicted ∆∆G are plotted in the Bar Chart.
The result table and Antibody-Antigen complex which of B-factor is replaced with average ∆∆G are downloadable through the Download section.
Alanine Scanning gives predicted ∆∆G for antibody-antigen interface residues. Same as the previous mutation list prediction, the result is shown in Result table, Visualisation and Bar Chart sections.
Apart from the Result and Visualisation sections, Saturation Mutagenesis shows a series of predicted ∆∆G for each of antibody-antigen interface reisdues using Heat Map.