| Active | Index | Mutation | Distance b/w mutants | Distance to interface | ΔΔGaffinity | Outcome |
|---|---|---|---|---|---|---|
| 0 | EL93W;VH24A | 13.28 | 5.64;15.9 | -0.66 | Decreased affinity | |
| 1 | YL86K;YH32A | 15.35 | 20.14;3.38 | -1.4 | Decreased affinity |
PI interaction
VDW
Hydrogen Bond
Ionic
Aromatic
Hydrophobic
Carbonyl
Polar